Description
PART I – Vasculogenic Mimicry Background 1. Vasculogenic Mimicry – An Overview Emily Marques dos Reis and Fernanda Vieira Berti 2. In vitro Models to Study Angiogenesis and Vasculature Ralph Francescone and Dbora Barbosa Vendramini-Costa PART II: Protocols for VM in vitro 3. In vitro Tube Formation Assays in Matrigel Ralph Francescone and Dbora Barbosa Vendramini-Costa 4. Vasculogenic mimicry in a 3D model in vitro Emily Marques dos Reis, Fernanda Vieira Berti, and Luismar Marques Porto 5. Cancer Cell Spheroids as a 3D Model for Exploring the Pathobiology of Vasculogenic Mimicry Mara de Andrade Peixoto, Emily Marques dos Reis, and Luismar Marques Porto 6. A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells Yarely M. Salinas-Vera, Dolores Gallardo-Rincn, Erika Ruz-Garca, Laurence A. Marchat, Jess Valdz, Carlos Vzquez-Calzada, and Csar Lpez-Camarillo 7. Co-Immunoprecipitation of Protein Complexes from Different Subcellular Compartments in Vasculogenic Mimicry Studies Daniel Delgado-Bellido, Angel Garcia-Diaz, and Francisco Javier Oliver PART III – Protocols for VM In Vivo 8. In vivo Models to Evaluate Antitumor Drugs Effect on Vasculogenic Mimicry Carlos E. Fonseca-Alves and Patrcia de F. Lainetti 9. Patient derived xenograft models for studying vascular mimicry in melanoma in vivo Manish Ranjan and Nobel Bhasin PART IV: VM Analysis 10. Histological evaluation of long-term collagen I culture Marcella Massimini, Mariarita Romanucci, Raffaella De Maria, and Leonardo Della Salda 11. Histochemical Staining of Vasculogenic Mimicry Saber Imani, Shuya Liu, Mazaher Maghsoudloo, and QingLian Wen 12. Identification of Vasculogenic Mimicry in Histological Samples Gustav Stlhammar 13. Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry in vitro Joseph Manarang and Alison McDermott 14. Immunohistochemistry for VM markers Alessio Biagioni and Elena Andreucci 15. Confocal laser microscopy for VM analysis with DAPI and phalloidin staining Karina Cesca and Eliana Medeiros Oliveira 16. Building Mathematical Models for Vascular Growth and Inhibition Fernanda Vieira Berti and Luismar Marques Porto
